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本文应用同位素标记物掺入法,观察紫外线对体外培养人胚肺二倍体成纤维细胞G_6HL株DNA合成的影响。结果表明,经不同剂量的紫外线照射后,不同生长阶段的人胚肺细胞的DNA合成能力各异。在较年轻的细胞,~3H-TdR掺入DNA的量大于对照组,而较老的细胞则低于对照组,且随着代数的增加,~3H-TdR掺入量下降越显著,同时呈现明显的剂量效应。提示紫外线照射对年轻细胞DNA合成似有促进作用,而对较老细胞则有显著的抑制作用,且细胞越老抑制作用越显著。说明随着细胞的衰老进程,细胞对外界环境的适应能力逐渐下降。这可能是导致衰老细胞容易死亡的原因之一。
In this paper, the effect of ultraviolet on the DNA synthesis of human embryonic lung diploid fibroblast G_6HL strain was observed by incorporation of isotope labeling. The results showed that the DNA synthesis ability of human embryonic lung cells at different stages of growth was different after irradiation with different doses of ultraviolet light. In younger cells, the amount of ~3H-TdR incorporated DNA was greater than that of the control group, while the older cells were lower than the control group. With the increase of the algebra, the amount of ~3H-TdR incorporation decreased more significantly. Obvious dose effect. It suggests that UV irradiation seems to promote the DNA synthesis of young cells, but it has a significant inhibitory effect on older cells, and the older the cells, the more significant the inhibitory effect. It shows that with the aging process of cells, the ability of cells to adapt to the external environment gradually declines. This may be one of the reasons that cause senescent cells to die.