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目的观察丹参酮IIA对大鼠系膜细胞增殖及对人TGF-β1基因启动子活性的作用。方法应用MTT法观察丹参酮IIA对大鼠系膜细胞增殖的作用;将不同长度的人TGF-β1基因启动子片段与氯霉素乙酰基转移酶(CAT)报告基因组成重组体phTGF 2.14,采用脂质体法转染至大鼠系膜细胞中,分别加入不同浓度的丹参酮IIA(1、5、10 mg/L)进行干预,用ELISA方法检测报告基因CAT的活性。结果10 mg/L的丹参酮IIA对大鼠肾小球系膜细胞增殖有明显的抑制作用,与对照组比较有明显差异(P<0.01)。丹参酮IIA浓度为1、5 mg/L时对重组体phTGF 2.14的表达活性无影响,当增大丹参酮IIA浓度为10 mg/L时对重组体phTGF2.14的表达活性有抑制作用,与对照组比较有显著差异(P<0.01)。结论丹参酮IIA能够抑制系膜细胞增殖,当浓度为10 mg/L时对人TGF-β1基因启动子活性也具有抑制作用。
Objective To observe the effect of tanshinone II A on rat mesangial cell proliferation and activity of human TGF-β1 promoter. Methods MTT assay was used to observe the effect of tanshinone II A on rat mesangial cell proliferation. Different lengths of human TGF-β1 gene promoter and chloramphenicol acetyltransferase (CAT) reporter gene were used to construct recombinant phTGF 2.14. The plastid method was transfected into rat mesangial cells, and different concentrations of tanshinone IIA (1, 5, 10 mg/L) were added to intervene. The activity of reporter gene CAT was detected by ELISA. Results 10 mg/L tanshinone IIA significantly inhibited the proliferation of rat glomerular mesangial cells, which was significantly different from that of the control group (P<0.01). When the concentration of tanshinone IIA was 1,5 mg/L, there was no effect on the expression activity of recombinant phTGF 2.14. When the concentration of tanshinone II A was 10 mg/L, the expression activity of recombinant phTGF2.14 was inhibited, compared with the control group. There were significant differences (P<0.01). Conclusion Tanshinone IIA can inhibit the proliferation of mesangial cells. When the concentration is 10 mg/L, it can also inhibit the promoter activity of human TGF-β1 gene.