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用膜片钳、反义寡核苷酸、免疫荧光及激光共聚焦显微镜等技术 ,研究MDR1基因在牛睫状体色素上皮(pigmentedciliaryepithelial,PCE)细胞容积激活性氯电流中的作用。PCE细胞表达MDR1基因产物 P糖蛋白 (P gp)。反义MDR1寡核苷酸抑制MDR1基因的表达 (P gp免疫荧光减少 93 % ) ,延缓容积激活性氯电流的出现 (潜伏期延长10 9% ) ,并导致激活率降低 62 %及电流峰值减小 5 6%。而核酸转染剂阳离子脂质体和非配对性的寡核苷酸对电流没有显著性影响。上述观察结果表明 ,睫状体色素上皮细胞容积激活性氯电流与内源性MDR1表达有关
The role of MDR1 gene in the cellular volume-activated chloride current in bovine ciliary body epithelial cells (PCE) was studied by patch clamp, antisense oligonucleotide, immunofluorescence and confocal laser scanning microscopy. PCE cells express the MDR1 gene product P-glycoprotein (Pgp). Antisense MDR1 oligonucleotides inhibited the expression of the MDR1 gene (93% reduction in P gp immunofluorescence), delayed the appearance of volume-activated chloride (10 9% latency increase) and resulted in a 62% reduction in activation and a decrease in current spikes 5 6%. The nucleic acid transfection agent cationic liposomes and unpaired oligonucleotides had no significant effect on the current. The above observations suggest that the volume-activated chloride current in ciliary body pigment epithelial cells is associated with endogenous MDR1 expression