论文部分内容阅读
从急性阿米巴痢疾患者粪便中分离所得的溶组织内阿米巴虫株SH-3、SH-6、SH-8的DNA和包囊携带者粪便中分离所得的溶组织内阿米巴 SH-5、SH-7的 DNA应用多聚酶链反应技术扩增30个周期后作琼脂糖凝胶电泳分析,发现致病性引物 P_(11)、P_(12)可使SH-8虫株的DNA增殖;而非致病性引物P_(13)、P_(14)可使SH-3、SH-5、SH-6和SH-7虫株的DNA增殖。另外,酶株群分析和单克隆抗体反应也显示SH-8为致病性虫株,而另4株虫株均属于非致病性虫株,与多聚酶链反应的结果相一致。提示,致病性虫株与非致病性虫株的基因有区别,多聚酶链反应对溶组织内阿米巴进行基因分析是一种敏感和特异的新方法。
The isolated E. histolytica strains SH-3, SH-6, and SH-8 from the stools of the patients with acute amebic dysentery were separated from the stools of the enolase of Entamoeba histolytica The DNA of SH-7 and SH-7 were amplified by polymerase chain reaction (PCR) and analyzed by agarose gel electrophoresis after 30 cycles. The results showed that the pathogenic primers P 11 and P 12 could make DNA of SH- While the non-pathogenic primers P 13 and P 14 could proliferate DNA of SH-3, SH-5, SH-6 and SH-7 strains. In addition, enzyme group analysis and monoclonal antibody reaction also showed SH-8 as pathogenic strains, while the other four strains belong to non-pathogenic strains, consistent with the results of polymerase chain reaction. Tip, pathogenic strains and non-pathogenic strains have different genes, polymerase chain reaction of Entamoeba histolytica gene analysis is a sensitive and specific new method.