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多沙唑嗪通常由中间体BCP和ACQ合成,为考察产品质量,有必要建立多沙唑嗪及其中间体的测定方法。该文建立并优化了反相高效液相色谱法(RP-HPLC)同时测定多沙唑嗪及其中间体。结果表明,以C18柱(4.6mm×250 mm)为色谱柱,流动相组成V(CH3OH)∶V[c(KH2PO4)=0.02 mol/L,pH=3.0]=70∶30,流速0.6 mL/min,检测波长246 nm,多沙唑嗪与BCP、ACQ可达到基线分离,分离度大于2.0。各物质在1~20 mg/L内的色谱峰面积与质量浓度之间线性关系良好(r>0.999),该方法的准确度(回收率>99%)和精密度(RSD<4%)高,操作简便快速,可望用于多沙唑嗪产品质量研究和控制。
Doxazosin is usually synthesized by the intermediates BCP and ACQ, in order to examine the product quality, it is necessary to establish the determination of doxazosin and its intermediates. This article established and optimized RP-HPLC simultaneous determination of doxazosin and its intermediates. The results showed that the mobile phase consisted of V (CH3OH): C (KH2PO4) = 0.02 mol / L, pH = 3.0] = 70:30 and the flow rate was 0.6 mL / min. The detection wavelength was 246 nm. Doxazosin and BCP and ACQ could be separated by baseline and the resolution was greater than 2.0. The linear relationship between chromatographic peak area and mass concentration of 1 ~ 20 mg / L was good (r> 0.999). The accuracy of this method (recovery> 99%) and precision (RSD <4%) were high , Easy to operate fast, is expected to be used for doxazosin product quality research and control.