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采用微量细胞培养法观察细胞病变效应 (Cytopathiceffect,CPE) ,测定经不同剂量大黄提取液作用后的病毒滴度 ,用逆转录—聚合酶链反应 (RT PCR)检测药物作用后细胞冻融液中的病毒核酸(CVB RNA) ,研究大黄提取液的抗柯萨奇病毒B组 (CVB)作用 ,探讨其抗病毒作用机制。结果 :不同剂量大黄提取液和病毒作用后 ,细胞均出现CPE ;不同剂量药物作用于细胞后 ,不能阻止CVB3感染 ;当CVB3感染细胞后 ,大黄提取液能阻止病毒增殖 ,并表现出一定的量效关系 ,其治疗指数(TI) =10。提示大黄提取液不能直接杀灭CVB3,不能阻止CVB3吸附、穿入易感细胞 ,而通过阻止CVB3核酸复制或 /和以后环节发挥抗病毒作用。
Cytopathic effect (CPE) was used to observe the cytopathic effect (CPE). The virus titer after different doses of rhubarb extract was measured. RT-PCR was used to detect the effect of drugs on the cell freeze-thaw. The virus nucleic acid (CVB RNA) was studied on the anti-CoxB virus (CVB) effect of Rhubarb extract and its antiviral mechanism was explored. Results: CPE was observed in cells after different doses of rhubarb extract and virus; different doses of drugs could not prevent CVB3 infection after the cells were applied; when CVB3 infected cells, rhubarb extract could prevent virus proliferation and showed a certain amount. Effect relationship, its therapeutic index (TI) =10. Rhubarb extract can not directly kill CVB3, can not prevent CVB3 adsorption, penetration into susceptible cells, and by preventing CVB3 nucleic acid replication or / and later links to play antiviral effect.