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为研究重离子辐照突变株的细胞遗传学变化,以碳离子辐照选育出的甜高粱早熟突变株KFJT-1及其对照品种KFJT-CK为材料,比较研究了两者之间花粉生活力大小以及花粉母细胞减数分裂的差异。结果发现:KFJT-1的花粉生活力以及花粉总数大于KFJT-CK;KFJT-1花粉母细胞减数分裂大多进入第二次减数分裂时,KFJT-CK花粉母细胞集中在第一次减数分裂;在KFJT-1减数分裂末期Ⅱ,出现了两组分裂时期不一致、3分体以及不均等分裂畸变状况,畸变率仅为4.5%,说明KFJT-1在生长繁殖过程中修复了大部分由于物理诱变造成的染色体畸变,且能稳定遗传。这些结果从生殖细胞水平进一步说明碳离子辐照早熟突变株KFJT-1较未突变株KFJT-CK成熟期早,且各项指标均优于KFJT-CK。
In order to study the cytogenetic changes of heavy ion-irradiated mutants, the early sweet-sorghum mutant KFJT-1 and its control KFJT-CK, which were bred by carbon ion irradiation, were used as materials to comparatively study pollen life The size of the force, and the difference in meiosis of pollen mother cells. The results showed that the pollen viability and the total number of pollen in KFJT-1 were higher than those in KFJT-CK. When meiosis of KFJT-1 mostly entered the second meiosis, KFJT-CK pollen mother cells concentrated in the first reduction At the late stage of KFJT-1 meiosis, there were two groups of incoordination, 3-split and non-uniform split-distortions at the split stage, with a rate of only 4.5%. This indicated that KFJT-1 repaired most of the growth and reproduction Chromosome aberration due to physical mutagenesis, and stable inheritance. These results further indicate that KFJT-1, which is a precursor of carbon ion irradiation, has earlier maturation stage than the non-mutant KFJT-CK at germ cell level, and all the indexes are better than KFJT-CK.