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目的探讨抗氧化应激是否参与参附注射液预处理诱导的肾脏保护作用。方法健康成年雄性SD大鼠21只随机分为假手术对照组(Sham组)、肾脏缺血再灌注组(I/R组)和参附注射液组(SF组);SF组给予参附注射液10mL/kg腹腔注射,每日1次,连续给药7d。麻醉下行右肾切除后,用无损伤动脉夹钳夹左侧肾蒂60min,再灌注24h,制备肾缺血再灌注损伤动物模型。比较各组SD大鼠再灌注24h肾脏组织中超氧化物歧化酶(superonidedismu tase,SOD)水平、过氧化氢酶(catalese,CAT)和丙二醛(malonical aldehyed,MDA)含量。结果与Sham组相比,I/R和SF组肾脏组织SOD和CAT显著降低,而MDA明显升高(P<0.05);与I/R组比,参附注射液能明显增加SOD和CAT水平(P<0.05),降低MDA含量(P<0.05)。结论参附注射液预处理可增强缺血再灌注损伤肾脏组织抗氧化应激,其表现为增强SOD和CAT的活力,减少MDA的生成。
Objective To investigate whether antioxidant stress is involved in the protective effect of Shenfu injection induced by renal preconditioning. Methods Twenty-one healthy adult male Sprague-Dawley rats were randomly divided into 4 groups: Sham group, I / R group and SF group. SF group was given Shenfu injection Fluid 10mL / kg intraperitoneal injection, 1 day, continuous administration of 7d. After anesthesia, the right kidney was excised and the left renal pedicle was clamped for 60 minutes and then perfused for 24 hours. The animal model of renal ischemia-reperfusion injury was prepared. The levels of superoxide dismutase (SOD), catalase (CAT) and malonaldehyde aldehyd (MDA) in kidneys of SD rats after reperfusion for 24 hours were compared. Results Compared with Sham group, SOD and CAT in kidney were significantly decreased and MDA in I / R and SF groups were significantly increased (P <0.05). Compared with I / R group, Shenfu Injection significantly increased the levels of SOD and CAT (P <0.05), and decreased MDA content (P <0.05). Conclusion Shenfu injection pretreatment can enhance the anti-oxidative stress in renal tissue after ischemia-reperfusion injury, and its performance is to enhance the activity of SOD and CAT and reduce the generation of MDA.