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目的 了解人眼巩膜成纤维细胞是否表达碱性成纤维细胞生长因子(bFGF)受体FGFR1和转化生长因子(TGF- β)受体TβRⅠ和TβRⅡ。方法 角膜移植后的4只眼球,应用定点解剖及游走促进法进行巩膜成纤维细胞的分离培养,建立细胞系,采用免疫荧光染色法检测bFGF受体FGFR1和TGF- β受体TβRⅠ和TβRⅡ蛋白的表达。结果 分别应用FGFR 1、TβRⅠ和TβRⅡ的特异多克隆抗体染色,整个细胞表面或细胞核周呈现特异性黄绿色荧光,受体位于细胞胞膜上。肉眼观察TβRⅠ和TβRⅡ呈强阳性表达,FGFR 1表达较弱于TβRⅠ和TβRⅡ。结论 人巩膜成纤维细胞表达bFGF受体FGFR1和TGF β受体TβRⅠ、TβR的功能性蛋白,巩膜是bFGF和TGF- β发挥作用的一个部位。外源的bFGF和TGF -β通过与巩膜成纤维细胞上的上述相应受体结合发挥作用,是影响实验性近视发生发展的机制之一。
Objective To investigate whether human scleral fibroblasts express basic fibroblast growth factor (bFGF) receptor FGFR1 and transforming growth factor (TGFβ) receptors TβRI and TβRII. Methods Four eyes after corneal transplantation were used to separate and culture scleral fibroblasts. The cell lines were established. The expression of FGFR1 and TβRⅡ of bFGF receptor and TGFβ receptor were detected by immunofluorescence staining expression. The results were stained with specific polyclonal antibodies against FGFR 1, TβRⅠ and TβRII, respectively. The whole cell surface or nuclei showed specific yellow-green fluorescence, and the receptor was located on the cell membrane. Macroscopically, TβRⅠ and TβRⅡwere strongly positive, whereas FGFR 1 expression was weaker than TβRⅠ and TβRⅡ. Conclusion Human scleral fibroblasts express the functional proteins of FGFR1 and TβRⅠ, TβR of TGFβ receptors. Sclera is a site where bFGF and TGF-β play a role. Exogenous bFGF and TGF-β through the scleral fibroblasts on the corresponding receptor binding play a role in the development of experimental myopia is one of the mechanisms.