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为了探明二化螟盘绒茧蜂Cotesia chilonis(Matsumura)寄生对寄主二化螟Chilo suppressalis(W alker)幼虫血细胞和体液免疫反应的影响,观察并测定了二化螟盘绒茧蜂寄生引起二化螟幼虫血细胞数量、延展、存活、吞噬和包囊作用以及血淋巴酚氧化酶活性等的变化。结果显示,二化螟幼虫血细胞总数在寄生后1天即显著高于对照。其中,颗粒血细胞和浆血细胞在寄生后的数量变化均与血细胞总数变化相似,颗粒血细胞在寄生后3天、浆血细胞在寄生后0.5天起即分别与其对照差异显著,但两种血细胞在血细胞总数中各自所占的比例与对照多无显著差异。二化螟幼虫血细胞延展能力在寄生后0.5天受到显著抑制,此后与对照无显著差异;同时寄生可增加寄主血细胞的死亡率。寄主幼虫血细胞的吞噬作用在寄生后2天起显著降低;包囊作用则在寄生后1天起显著降低。二化螟幼虫被二化螟盘绒茧蜂寄生后,血淋巴酚氧化酶活性显著升高,但随后呈逐渐下降趋势。研究结果说明,二化螟盘绒茧蜂寄生使寄主二化螟幼虫的免疫反应呈现一定的规律变化。
In order to investigate the influence of parasitism of Cotesia chilonis (Matsumura) on hemocyte and humoral immune responses to Chilo suppressalis (W alker) larvae, we observed and determined the parasitism The change of hemocyte number, extension, survival, phagocytosis and cyst and hemolymph phenoloxidase activity in the larvae of the rice borer. The results showed that the total number of larval hemolymph of S. striatus was significantly higher than that of the control one day after the parasitism. Among them, the changes in the number of granulocytes and plasma cells after the parasitization were similar to the changes in the total number of blood cells. The granulocytes were significantly different from the control at day 0 after parasitization, at 3 days after the parasitization. However, In their respective proportions and no significant difference between the control. The larval squamous larvae ’s blood cell extensibility was significantly inhibited at 0.5 days after the parasitism, and no significant difference with the control afterwards; parasitism could increase the death rate of host blood cells. Phagocytosis of host larval blood cells decreased significantly from 2 days after parasitization; the cyst effect was significantly reduced from day 1 after parasitism. After the larvae of the rice stem borer were parasitized by the Chilo suppressalis, the hemolymph phenoloxidase activity increased significantly, but then decreased gradually. The results showed that the parasitism of C. borealis caused some changes in the immune response of the S. inferens larvae.