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目的观察急性一氧化碳(CO)中毒后纹状体多巴胺(DA)及其代谢产物含量的动态变化,探讨单胺氧化酶B(MAO-B)及DA系统变化在急性CO中毒迟发性脑病(delayed neruopsychologic sequelae,DNS)发病中的意义。方法采用腹腔注射CO法制备急性CO中毒大鼠模型,利用阿朴吗啡诱导旋转实验鉴定DNS模型,分析不同模型脑组织纹状体DA系统、MAO-B活性变化。结果急性CO中毒后纹状体MAO-B活性即明显降低,仅为对照组水平的43%;同时用清醒动物脑微透析实验观察纹状体DA及其代谢产物浓度变化发现,CO中毒后1 h DA浓度即增加,其代谢产物则明显降低,提示急性CO中毒早期DA代谢显著降低;中毒14 d后DNS模型大鼠纹状体MAO-B活性显著增加,为对照组活性的1.7倍,DA浓度则显著降低,仅为对照组的29%,代谢产物浓度明显增加,提示出现运动功能障碍的DNS模型脑纹状体MAO-B活性显著升高,DA代谢明显增加。结论急性CO中毒DNS大鼠脑纹状体MAO-B活性可异常升高,加速DA代谢,影响DA神经系统保持锥体及锥体外系运动协调功能,同时生成的大量DA代谢产物具有毒性作用,可进一步加重脑循环障碍和氧化应激损伤,形成恶性循环。
Objective To investigate the dynamic changes of dopamine (DA) and its metabolites in striatum after acute carbon monoxide (CO) poisoning and to investigate the changes of monoamine oxidase B (MAO-B) and DA system in patients with delayed neruopsychologic sequelae DNS) the significance of the onset. Methods The rat model of acute CO poisoning was established by intraperitoneal injection of CO. DNS model was established by apomorphine induced rotation test, and the activity of DAO and MAO-B in striatum of different models were analyzed. Results The activity of MAO-B in striatum was significantly decreased after acute CO poisoning, which was only 43% of that in the control group. Simultaneously, the concentration of DA and its metabolite in striatum was observed with brain microdialysis in conscious animals. h DA concentration increased significantly, the metabolites were significantly reduced, suggesting that acute CO poisoning early DA metabolism was significantly reduced; 14 d poisoning DNS rat striatum MAO-B activity was significantly increased 1.7 times the activity of the control group, DA Concentration was significantly reduced, only 29% of the control group, the metabolite concentration was significantly increased, suggesting that motor function disorder in the DNS model of striatum MAO-B activity was significantly increased DA metabolites increased significantly. Conclusions The activity of MAO-B in the striatum of the acute CO poisoning rats can be abnormally increased, the metabolism of DA may be accelerated, the coordination function of the nervous system in the pyramidal neurons and the extrapyramidal motor system will be affected. At the same time, a large amount of metabolites of DA produced may have toxic effects, Can further aggravate cerebral circulation disorder and oxidative stress injury, forming a vicious circle.