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目的:探讨刺五加对睡眠剥夺果蝇睡眠的改善作用,并进行差异基因表达谱初步分析。方法:在恒温、恒湿、自动光控条件下,选取7日龄野生型Canton S品系黑腹果蝇为试验对象,采用光照刺激的方法复制果蝇睡眠剥夺模型,利用自动红外果蝇活动监测系统,以果蝇24h总睡眠时间和睡眠指数为观察指标,进行刺五加改善睡眠剥夺果蝇睡眠作用的量-效与时-效关系研究;在此基础上,采用果蝇表达谱芯片技术进行刺五加改善果蝇睡眠的差异基因表达谱初步分析。结果:刺五加浓度1%、给药3天以上,对果蝇睡眠的改善作用最明显;与模型组相比,刺五加组共有差异表达基因685个,上调基因188个,下调基因497个。结论:刺五加对果蝇睡眠剥夺模型的睡眠具有明显的改善作用,其作用机制涉及免疫反应和多细胞生物过程等相关基因表达的变化。
Objective: To investigate the effects of Acanthopanax senticosus on the sleep deprivation of Drosophila melanogaster, and preliminary analysis of differential gene expression profiles. Methods: Drosophila melanogaster Drosophila melanogaster, a 7-day-old wild type strain, was selected under the conditions of constant temperature, constant humidity and automatic light control. Drosophila sleep deprivation model was replicated by light stimulation and monitored by automatic infrared fly activity monitoring System, the Drosophila 24h total sleep time and sleep index as the observation index, the Acanthopanax senticosus to improve the sleep-deprived Drosophila sleep effect of dose-effect and time-effectiveness relationship; on this basis, using Drosophila expression profiling chip technology Preliminary analysis of differential gene expression profile of Acanthopanax senticosus to improve Drosophila sleep. Results: The acanthopanax senticosus at a concentration of 1% administered more than 3 days had the most obvious effect on Drosophila sleep. Compared with the model group, there were 685 differentially expressed genes in Acanthopanax senticosus group with 188 up-regulated genes and 497 down-regulated genes A Conclusion: Acanthopanax senticosus significantly improves the sleep of Drosophila sleep deprivation model, and its mechanism of action involves the changes of related genes such as immune response and multicellular biological processes.