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用微量凯氏定氮法,对根结线虫(Meloidogyneincognita)的寄主番茄上的根结和同株上的健根以及健株的健根蛋白质含量测定表明:寄主在整个生长期间,根结内蛋白质含量在一定的时期内,显著高于同期同株健根,也高于同期相似部位的健株健根,且根结中蛋白质含量变化也都是由低到高再到低,即接种时间越长其蛋白质含量越多;同株根结与健株中的蛋白质含量的差值在寄主生长的前中期是逐渐拉大的,根结开始腐烂后,蛋白质含量开始下降。但病株和健株蛋白质总量(包括根、茎、叶和果实)相比,前者则显著低于后者。另用桂花树根上的根结(由Meloidogyneincognita感染)和一年生野生寄主龙葵(Blacknightshacle,由Meloidogynejavanica感染)即不同寄主和不同的根结线虫种类测定,结果同上。
Micro-Kjeldahl method was applied to determine the root tuber and root tuber of the root tuber of Meloidogyne incognita on the same plant and healthy root tuber. The results showed that during the whole growth period, Content in a certain period of time, significantly higher than the same period of the same root, but also higher than similar parts of the same period healthy roots, and the root knot protein content changes are from low to high and then low, that the time of inoculation The longer the protein content; the same amount of root knot and healthy plant protein content difference between the host growth in the early and middle is gradually widened, the root knot began to decay, the protein content began to decline. However, compared with the total amount of plant protein (including roots, stems, leaves and fruits), the former was significantly lower than the latter. In addition, the root knots (infected by Meloidogyne incognita) and the annual wild host (Blacknightshacle, infected by Meloidogynejavanica) on sweet-scented osmanthus roots were determined by different hosts and different root-knot nematode species.