论文部分内容阅读
目的:建立健康产品(食品、保健品、药品)中非法添加利巴韦林的快速筛查及确证方法。方法:采用分散固相萃取-高效液相色谱(HPLC)快速筛查抗病毒药品。样品(胶囊、冲剂、片剂、口服液均可)提取后,加入固相萃取填料净化,采用Phenomenex强阳离子氢型交换色谱柱(300 mm×7.8 mm),以水(稀硫酸调至p H 2.5±0.1)为流动相,流速0.5 m L/min,柱温60℃,检测波长207 nm。阳性样品采用液相色谱-串联质谱(LC-MS/MS)进行确证。结果:利巴韦林在0.01~0.30 mg/m L浓度范围内线性关系良好(r=0.999 7),加样回收率为100.9%,RSD为0.8%。结论:该方法可以简便、准确、灵敏地测定健康产品中非法添加的利巴韦林。
OBJECTIVE: To establish a rapid screening and confirmatory method of illegally adding ribavirin to health products (food, health products, medicines). Methods: Antiviral drugs were rapidly screened by dispersive solid phase extraction - high performance liquid chromatography (HPLC). The samples (capsules, granules, tablets and oral solution) can be extracted and then purified by solid-phase extraction (SPE) using a Phenomenex strong cation exchange column (300 mm × 7.8 mm) 2.5 ± 0.1) as mobile phase at a flow rate of 0.5 m L / min. The column temperature was 60 ℃ and the detection wavelength was 207 nm. Positive samples were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS / MS). Results: Ribavirin had a good linearity (r = 0.999 7) within the range of 0.01-0.30 mg / m L with a recovery of 100.9% and a RSD of 0.8%. Conclusion: This method allows for the easy, accurate and sensitive determination of ribavirin illegally added to health products.