从溶组织内阿米巴HM-1:IMSS株抽提的DNA,用聚合酶链反应(PCR)扩增此虫株的基因序列,对其肌球蛋白重链基因(mh-cA)进行鉴定,并分析其核苷酸序列和RNA。结果显示,源于HM-1:IMSS虫株的mhcA基因存在于溶组织内阿米巴中,其编码蛋白质的N端区域与其他原虫具有高度的同源性,但象其他已鉴定的基因不含内含子。用这种阿米巴的基因组DNA作PCR,扩增产物用聚丙烯酰胺凝胶电泳分析,显示有 The mtDNA was extracted from the DNA of Mycobacterium Entamoeba histolytica HM-1: IMSS strain and its gene sequence was amplified by polymerase chain reaction (PCR), and its myosin heavy chain gene (mh-cA) was identified , And analyzed its nucleotide sequence and RNA. The results showed that the mhcA gene from the HM-1: IMSS strain was present in Entamoeba histolytica and its N-terminal region encoding the protein had high homology with other protozoa, but unlike other protozoa Intron. Using this genomic DNA of Amoeba for PCR, the amplified product was analyzed by polyacrylamide gel electrophoresis and showed that there was