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目的测定北社鼠对杀鼠灵的敏感性,为检验该鼠的抗药性提供参数。方法实验室做单个笼养无选择摄食试验:雌雄分开,随机分组,按5个不同食毒期供给浓度为0.025%的杀鼠灵毒饵;用Bliss计算机软件,计算不同食毒期与对应死亡率之间的关系。结果北社鼠对杀鼠灵的敏感性,性别之间差异无统计学意义(P_b>0.05,P_(LFP50)>0.05);故将雌雄数据合并计算,得毒力回归方程y=3.29+4.33x,LFP_(50)、LFP_(95)、LF_(99)及其95%置信限分别为2.48(1.97～3.13)、5.96(3.92～9.06)和8.56(4.65～15.78)d。结论按世界卫生组织推荐的以致死99%敏感靶标鼠的食毒期(LF_(90))取整天数作为抗药性检验标准,山西省娄烦地区北社鼠对0.025%的杀鼠灵毒饵摄食期超过9 d存活即为抗性鼠。
Objective To determine the sensitivity of Beishe mouse to warfarin and provide parameters for testing its resistance. Methods The experiment was conducted in a single captive selective feeding experiment: male and female were randomly divided into groups and fed with warfarin at a concentration of 0.025% at five different feeding stages. Bliss computer software was used to calculate the relative mortality The relationship between. Results There was no significant difference in the sensitivity and the sex between the two groups (P_b> 0.05, P_ (LFP50)> 0.05). Therefore, the male and female data were combined to calculate the virulence regression equation y = 3.29 + 4.33 The LFP 50, LFP 95, LF 99 and their 95% confidence intervals were 2.48 (1.97 to 3.13), 5.96 (3.92 to 9.06) and 8.56 (4.65 to 15.78) d, respectively. Conclusion According to World Health Organization recommended mortality rate of 99% of the sensitive target mice (LF_ (90)) taking the whole day as the drug resistance test standard, Loufan area of Shanxi Province North Society of 0.025% of the warfarin poison bait Feeding for more than 9 d survival is resistant mice.