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目的:观察尼莫地平对实验性颅脑损伤后神经细胞胞质内游离钙的作用.方法:用可监控打击强度的流体冲击致脑损伤装置使大鼠中度脑损伤,荧光指示剂Fura-2/AM标记,检测脑损伤后脑海马胞质内游离钙的浓度.伤后用尼莫地平(0.04mg/kg,iv)处理.结果:脑损伤后胞质内游离钙明显增加.尼莫地平处理后明显地降低胞质内游离钙的浓度(P<0.01).结论:尼莫地平可明显地阻断神经细胞膜上钙通道,减少钙内流,减轻脑损伤后钙内流对细胞造成的损害.
Objective: To observe the effect of nimodipine on intracellular free calcium in neurons after experimental craniocerebral injury.Methods: The rats were subjected to fluid-induced brain injury with fluid shock to monitor the intensity of injury, and the fluorescence indicator Fura- 2 / AM was used to detect the concentration of intracellular free calcium in brain hippocampus after brain injury.Nimodipine (0.04mg / kg, iv) was used after injury.Results: After intracerebral injury, intracellular free calcium was significantly increased.Nimodipine (P <0.01) .Conclusion: Nimodipine can obviously block the calcium channels in the nerve cell membrane, reduce the influx of calcium and reduce the calcium influx in the cells after brain injury damage.