为改善直接法中期相数量和质量,作者设计了载玻片制作仪。基于该仪器的使用,建立了一种只要能使每例至少得到5mg标本就能获得大量好的带和可分析的中期相的方法。在CVS后尽快将带有镜下可见柱状结构的完整绒毛在37℃Hank′s液中清洗。将10～20mg的绒毛标本移入盛有含5%子牛血清的3ml RPMI— To improve the quantity and quality of direct phase mesophase, the author designed a slide maker. Based on the use of this instrument, a method was developed that produces a large number of good bands and an analyzable metaphase as long as at least 5 mg of each sample is obtained. Immediately after CVS, intact villi with microscopically visible columnar structures were washed in Hank’s solution at 37 ° C. 10-20 mg of the villus specimen was transferred to a 3 ml RPMI-