论文部分内容阅读
目的:建立基于可调纳米结构的表面增强拉曼(SERS)特性的DNA检测新方法。方法:通过电化学沉积在ITO导电玻璃表面制备形貌可控的纳米金结构,用拉曼活性分子尼罗蓝A(NBA)表征其拉曼特性,再用于对沙门氏菌目标DNA的检测。结果:纳米金结构形貌可通过电沉积过程中的沉积电压和沉积时间调控,结构基底对NBA和DNA分子均有良好的SERS活性。结论:电化学沉积制备的纳米结构具有良好的均一性、重复性和稳定性,是1种理想的SERS基底,可用于生物分子DNA的拉曼检测。
Objective: To establish a new DNA detection method based on surface-enhanced Raman (SERS) properties with tunable nanostructures. Methods: The nanostructured gold nanostructures with controlled morphology were prepared by electrochemical deposition on the surface of ITO conductive glass. The Raman properties of the nanostructured nanostructures were characterized by Raman-active molecule Nile Blue A (NBA), which was used to detect the target DNA of Salmonella. Results: The morphology of Au nanostructures was controlled by the deposition voltage and deposition time in the electrodeposition process. The structural substrates exhibited good SERS activity for both NBA and DNA molecules. Conclusion: The nanostructures prepared by electrochemical deposition have good uniformity, repeatability and stability. They are ideal SERS substrates for Raman detection of biomolecule DNA.