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目的:探索大黄虫丸对肺纤维化形成阶段的干预作用机制。方法:将48只普通级健康wistar大鼠用平阳霉素复制肺纤维化模型大鼠后,分为正常对照组、模型对照组、大黄虫丸组和强的松对照组。大黄虫丸剂量1.2 g.kg-1,从第14 d开始按15 mL.kg-1体重ig,造模28 d后处死,测定、比较各组大鼠血清和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中超氧化物歧化酶(superoxide dismutase,SOD)活性及丙二醛(malondialdehyde,MDA)含量。结果:模型组大鼠血清及BALF中SOD活性低于正常对照组、MDA含量高于正常对照组,大黄虫丸组SOD活性均高于模型组,MDA含量均低于模型组;大鼠BALF中SOD活性、MDA含量与血清SOD活性、MDA含量呈正相关。结论:大黄虫丸有抑制模型大鼠肺及血清中SOD活性下降及MDA含量增高的作用;血清SOD活性和MDA含量可间接反映肺部脂质过氧化与抗氧化水平。
Objective: To explore the mechanism of intervention of Rhubarb Zhechong Pills on the formation of pulmonary fibrosis. Methods: Forty-eight healthy wistar rats were randomly divided into normal control group, model control group, rhubarb and cholestatic pill group and prednisone control group. Rhubarb worm pill dose of 1.2 g.kg-1, starting from the fourteenth day according to 15 mL.kg-1 body weight ig, 28 days after the model was sacrificed, determination, comparison of rat serum and bronchoalveolar lavage fluid (bronchoalveolar (superoxide dismutase, SOD) and malondialdehyde (MDA) content in lavage fluid and BALF. Results: The activity of SOD in serum and BALF in model group was lower than that in normal control group. The content of MDA in model group was higher than that in normal control group. The activity of SOD in Rhubarb group was higher than that in model group. The content of MDA in model group was lower than that in model group. In the SOD activity, MDA content and serum SOD activity, MDA content was positively correlated. Conclusion: Dahuang Zhechong Pill can inhibit the decrease of SOD activity and the increase of MDA in the lung and serum of rats, and the serum SOD activity and MDA content indirectly reflect the level of lipid peroxidation and antioxidation in the lungs.