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目的:建立同时测定痰热清注射液中绿原酸、黄芩苷、熊去氧胆酸含量和鹅去氧胆酸限量的高效液相色谱法。方法:色谱条件:Agilent Zorbax SB-C18柱;流动相为0.15%醋酸溶液-0.15%醋酸乙腈,线性梯度洗脱,流速为0.8 mL.min-1;柱温为30℃;紫外检测波长:绿原酸326 nm,黄芩苷276 nm;蒸发光散射检测器漂移管温度为100℃,雾化气流速为1.5 L.min-1。结果:绿原酸、黄芩苷、熊去氧胆酸和鹅去氧胆酸分别在0.221～4.42,20.3～406,19.8～396,17.9～179 mg.L-1质量浓度范围内呈良好的线性关系,r均大于0.9995;平均加样回收率分别为101%,98.4%,99.4%,102%;4种化合物含量重复性试验RSD小于1.8%。结论:该方法简便、准确,重复性好,在对痰热清注射液中主要有效成分绿原酸、黄芩苷、熊去氧胆酸进行定量的同时,实现对鹅去氧胆酸限量的测定。
Objective: To establish a simultaneous determination of chlorogenic acid, baicalin, ursodeoxycholic acid and chenodeoxycholic acid in Tanreqing Injection high performance liquid chromatography. Method: The chromatographic conditions were Agilent Zorbax SB-C18 column with a mobile phase of 0.15% acetic acid-0.15% acetic acid in acetonitrile with a linear gradient of 0.8 mL · min-1. The column temperature was 30 ℃. UV detection wavelength: The original acid was 326 nm and baicalin was 276 nm. The drift tube temperature of the evaporative light scattering detector was 100 ℃, and the atomization gas flow rate was 1.5 L.min-1. Results: Chlorogenic acid, baicalin, ursodeoxycholic acid and chenodeoxycholic acid showed good linearity in the range of 0.221 ~ 4.42,20.3 ~ 406,19.8 ~ 396,17.9 ~ 179 mg.L-1, respectively The correlation and r were all greater than 0.9995. The average recoveries were 101%, 98.4%, 99.4% and 102%, respectively. The repeatability of four compounds was less than 1.8% RSD. Conclusion: The method is simple, accurate and reproducible. The quantitative determination of chlorogenic acid, baicalin and ursodeoxycholic acid in Tanreqing Injection is the most effective method for the determination of chenodeoxycholic acid .