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目的:探讨鞣花酸在大鼠不同肠段的吸收动力学特征及吸收机制。方法:采用大鼠在体小肠单向灌流实验模型,以高效液相色谱法测定鞣花酸药物浓度,分别研究不同药物浓度,不同吸收部位对鞣花酸大鼠肠吸收的影响。结果:鞣花酸在6~18μg·ml-1内时,相同肠段在不同浓度,吸收速率常数(Ka)及有效渗透系数(Peff)差异无显著性(P>0.05);不同肠段在相同浓度,结肠Ka及Peff差异有显著性(P<0.05),为特异性吸收,其他3个肠段差异无显著性;鞣花酸的Peff>1.2×10-3 cm·min-1,表明其属于高渗透性药物。结论:鞣花酸浓度对Ka及Peff无显著性影响;鞣花酸的肠道最佳吸收部位为结肠;鞣花酸在大鼠肠道的吸收机制为被动扩散。
Objective: To investigate the absorption kinetics and mechanism of ellagic acid in different intestine of rats. Methods: The rat model of intestine intestinal perfusion was used to determine the drug concentration of ellagic acid by high performance liquid chromatography (HPLC). The effects of different concentration of drug and different absorption sites on intestinal absorption of ellagic acid were studied. Results: When the ellagic acid was in the range of 6 ~ 18μg · ml-1, there was no significant difference in the absorption rate constant (Ka) and effective permeability coefficient (Peff) between the same intestinal segments at different concentrations (P> 0.05) At the same concentration, there was significant difference between Ka and Peff in colon (P <0.05), which was the specific absorption, while there was no significant difference in the other three bowel segments. The Peff of ellagic acid was> 1.2 × 10-3 cm · min-1 It is a highly permeable drug. CONCLUSION: The ellagic acid concentration has no significant effect on Ka and Peff. The optimal absorption site of ellagic acid in intestine is colon. The ellagic acid absorption mechanism in rat intestine is passive diffusion.