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To reveal the insecticidal mechanism of terpinen-4-ol,the activity of Na+,K+-ATPase in insects tested were determined in vivo and in vitro.The results showed that terpinen-4-ol and its ester derivatives had strong contact activity to housefly and the contact toxicities of its derivatives except Z3 were all superior or equivalent to terpinen-4-ol.All the 7 compounds had strong inhibition towards activity of Na+,K+-ATPase.With poisoning symptom exacerbating,the inhibition rates were gradually increased.In vitro,the IC50 of terpinen-4-ol,Z1,Z2,Z4,Z5,and Z6 was 155.89,197.98,96.02,121.36,124.85,and 153.74 μg mL-1,respectively.There was well correlation between the LD50 of terpinen-4-ol derivatives to housefly and the IC50 of terpinen-4-ol derivatives to Na+,K+-ATPase in housefly.In conclusion,Na+,K+-ATPase was likely the target of terpinen-4-ol against insects.
To reveal the insecticidal mechanism of terpinen-4-ol, the activity of Na +, K + -ATPase in insects tested were determined in vivo and in vitro. The results showed that terpinen-4-ol and its ester derivatives had strong contact activity to housefly and the contact toxicities of its derivatives except Z3 were all superior or equivalent to terpinen-4-ol. All the 7 compounds had strong inhibition towards activity of Na +, K + -ATPase. Whilst the poisoning symptom exacerbating, the inhibition rates were increasingly increased. The IC50 of terpinen-4-ol, Z1, Z2, Z4, Z5, and Z6 was 155.89, 197.98, 96.02, 121.36, 124.85, and 153.74 μg mL-1, respectively. Well was well correlation between the LD50 of terpinen -4-ol derivatives to housefly and the IC50 of terpinen-4-ol derivatives to Na +, K + -ATPase in housefly. In conclusion, Na +, K + -ATPase was likely the target of terpinen-4-ol against insects.