论文部分内容阅读
对冀东地区收集的162份野生大豆(Glycine soja)材料进行大豆花叶病毒(SMV)抗性鉴定并对筛选到的抗、感材料植株体内的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性及病程相关基因GmPR-1和GmPR-10的表达进行测定。结果表明:抗病野生大豆材料7份(4.3%),中抗材料6份(3.7%),中感材料62份(38.3%),感病材料87份(53.7%);抗病材料POD和CAT活性比对照显著增加,而感病材料显著降低,抗病材料的GmPR-1和GmPR-10基因表达量比对照显著增加,感病材料与对照无显著差异,初步表明POD、CAT及病程相关基因GmPR-1和GmPR-10可能与大豆抗病性有关。
162 soybean (Glycine soja) collected in the eastern part of Hebei Province were identified for their resistance to soybean mosaic virus (SMV) and their activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activity and the expression of GmPR-1 and GmPR-10 were detected. The results showed that 7 (4.3%) resistant soybean materials, 6 anti-disease materials (3.7%), 62 susceptible materials (38.3%) and susceptible materials 87 (53.7% CAT activity significantly increased compared with the control, while the susceptible materials significantly decreased, GmPR-1 and GmPR-10 gene expression of disease-resistant material significantly increased compared with the control, susceptible materials and the control no significant difference, preliminary POD, CAT and duration of the disease The genes GmPR-1 and GmPR-10 may be related to soybean disease resistance.