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背景:国内外动物实验已证实超声联合微泡能够显著增强干细胞移植治疗缺血性心血管疾病,但对其作用机制仍不明确目的:通过超声微泡体外作用于细胞,探讨超声联合微泡能够显著增强干细胞移植治疗缺血性心血管疾病的机制。方法:体外分别培养大鼠血管内皮细胞和骨髓间充质干细胞于培养板中,随机各分为对照组、超声组、超声微泡组。对照组不做干预,超声组以频率1 MHz,输出功率1 W/cm2,持续辐照90 s;超声微泡组加入5μL含氟碳气体脂质体超声微泡(约2×1011L-1),以同样超声条件作用90 s。结果与结论:超声微泡组血管内皮细胞上清液的血管内皮生长因子和基质细胞衍生因子1表达水平均比对照组显著增高(P<0.05)。超声组血管内皮生长因子表达水平为与对照组无差异;而基质细胞衍生因子1为较对照组明显降低(P<0.01)。骨髓间充质干细胞干预后CXCR4基因表达,超声微泡组、超声组较对照组均明显增强(P<0.01),但超声微泡组和超声组无统计学差异(P>0.05)。说明超声(1 MHz,1 W/cm2)联合微泡作用90 s能够促进血管内皮细胞分泌细胞因子(血管内皮生长因子和基质细胞衍生因子1),同时促进骨髓间充质干细胞CXCR4基因表达,是其增强移植干细胞归巢效应的机制之一。
BACKGROUND: Animal experiments at home and abroad have confirmed that ultrasound combined with microbubbles can significantly enhance stem cell transplantation in the treatment of ischemic cardiovascular disease, but its mechanism remains unclear. OBJECTIVE: To investigate the effect of ultrasound combined with microbubbles Significantly enhance the mechanism of stem cell transplantation in the treatment of ischemic cardiovascular disease. Methods: Rat vascular endothelial cells and bone marrow mesenchymal stem cells were cultured in vitro and divided into control group, ultrasound group and ultrasound microbubble group randomly. In the control group, the ultrasound group was irradiated with ultrasound at a frequency of 1 MHz and an output power of 1 W / cm2 for 90 s. Ultrasound microbubbles group was added with 5 μL of fluorocarbon-containing liposomes (about 2 × 10 11 L -1) , With the same ultrasonic conditions for 90 s. RESULTS AND CONCLUSION: Vascular endothelial growth factor and stromal cell-derived factor-1 expression in vascular endothelial cell supernatant of ultrasound microbubbles group were significantly higher than those of control group (P <0.05). The expression level of vascular endothelial growth factor in the ultrasound group was not different from that in the control group; while the stromal cell-derived factor 1 was significantly lower than that in the control group (P <0.01). The expression of CXCR4 gene in bone marrow mesenchymal stem cells (MSCs) group was significantly higher than that in control group (P <0.01), but there was no significant difference between ultrasound microbubbles group and ultrasound group (P> 0.05). These results suggest that ultrasound (1 MHz, 1 W / cm2) combined with microbubbles for 90 s can promote the secretion of cytokines (vascular endothelial growth factor and stromal cell-derived factor 1) by vascular endothelial cells and promote the expression of CXCR4 gene in bone marrow mesenchymal stem cells It is one of the mechanisms that enhances homing effect of transplanted stem cells.