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目的 探讨成肌细胞连续传代能力 ,选择适宜肌组织工程研究的成肌细胞。方法 常规传代培养人胚骨骼肌成肌细胞 ,以生长曲线、融合率分别观察细胞增殖、分化能力 ,探讨成纤维细胞沾染对传代细胞的影响。结果 第 6代以内细胞成纤维细胞沾染轻 ,主要表现出成肌细胞的生长特性 ,增殖较旺盛 ,分化能力高。第 8代～第 16代细胞成纤维细胞沾染重 ,表现出成纤维细胞的生长特性 ,增殖速度明显加快但分化能力低。第 2 0代细胞退变明显 ,细胞增殖与分化能力均丧失。结论 第 6代以内细胞生长特性以成肌细胞为主 ,增殖能力强 ,肌小管形成率高 ,适宜于组织工程研究。
Objective To investigate the continuous passage ability of myoblasts and to select myoblasts suitable for muscle tissue engineering research. Methods Human embryonic skeletal muscle myoblasts were routinely subcultured. The proliferation and differentiation of human skeletal muscle myoblasts were observed by growth curve and fusion rate respectively. The effects of fibroblast contamination on the passaged cells were investigated. Results The 6th generation of cells infected with fibroblasts light, mainly showing the growth characteristics of myoblasts, proliferation more vigorous, high differentiation capacity. The 8th to the 16th generation cells were infected with fibroblasts, showing the growth characteristics of fibroblasts, the proliferation rate was significantly accelerated but the differentiation ability was low. The 20th generation of cells degenerated significantly, cell proliferation and differentiation were lost. Conclusion The cell growth characteristics in the 6th generation are mainly myoblasts with strong proliferative ability and high rate of myotubes formation, which is suitable for tissue engineering research.