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目的 筛选并鉴定埃及伊蚊（Aedes aegypti）全基因组中的丝氨酸蛋白酶抑制蛋白（serine protease inhibitor, serpin）基因，分析其基因结构以及在不同发育时期和不同组织中的表达谱。 方法 运用生物信息学方法在埃及伊蚊全基因组数据库中筛选并鉴定埃及伊蚊serpin基因家族，利用DNAstar和Vector NTI11.0软件进行同源性比对、保守性分析，采用MEGA7.0软件构建系统进化树。实时荧光定量PCR（qRT?鄄PCR）检测埃及伊蚊不同发育时期（卵、Ⅰ～Ⅳ龄幼虫、蛹、雄蚊和未吸血雌蚊），以及未吸血雌蚊不同组织（唾液腺、中肠、脂肪体和卵巢）的serpin基因表达变化。 结果 从埃及伊蚊全基因组中筛选出26条serpin基因序列。生物信息学分析结果显示，19条具有信号肽，16条推测具有抑制酶活性的作用。进化分析表明，埃及伊蚊serpin基因家族被分为A、B和C分支，B分支分为4组，每分支均具有同源性，且不同成员间在进化上相对保守。qRT?鄄PCR结果显示，AaSRPN25在雌蚊中特异性表达，相对表达量为0.074 ± 0.015（P < 0.01）；AaSRPN19～22在雄蚊中丰富表达；AaSRPN23在Ⅰ龄幼虫时期特异性高表达，相对表达量为22.9 ± 5.28（P < 0.01）；AaSRPN23、AaSRPN25在唾液腺中特异性高表达，相对表达量分别为4.244 ± 1.39和74.44 ± 25.76（P < 0.01），AaSRPN6、AaSRPN14在中肠中的表达量最高，相对表达量分别为1.80 ± 0.33、0.703 ± 0.501；AaSRPN17、AaSRPN20在脂肪体中的表达量最高，相对表达量分别为0.34 ± 0.09、0.15 ± 0.03。 结论 从埃及伊蚊全基因组中筛选出26条serpin基因序列，其中19条具有信号肽，16条推测具有抑制酶活性的作用。
Objective To screen and identify the serine protease inhibitor (serpin) gene in the genome of Aedes aegypti and analyze its gene structure and expression profiles in different developmental stages and tissues. Methods The serpin gene family of Aedes aegypti was screened and identified by using bioinformatics method in the genome database of Aedes aegypti. DNAstar and Vector NTI11.0 software were used for homology comparison and conservative analysis. MEGA7.0 software was used to construct the system Evolutionary tree. Real-time quantitative PCR (qRT? -PCR) was used to detect the different developmental stages of the Aedes aegypti (eggs, larvae of the first to fourth instar, pupae, male and non-sucking females) Fat body and ovary) serpin gene expression changes. Results A total of 26 serpin genes were screened from the Aedes aegypti genome. Bioinformatics analysis showed that 19 of them had signal peptide and 16 of them had the suppressive effect of inhibiting enzyme activity. Phylogenetic analysis showed that the serpin gene family of Aedes aegypti was divided into A, B and C branches. B branches were divided into 4 groups, each branch shared homology, and the evolutionary relationships among different members were relatively conservative. qRT-PCR results showed that AaSRPN25 was specifically expressed in female mosquitoes, the relative expression level was 0.074 ± 0.015 (P <0.01); AaSRPN19-22 was highly expressed in male mosquitoes; AaSRPN23 was highly expressed in the first instar larvae , Relative expression level was 22.9 ± 5.28 (P <0.01). AaSRPN23 and AaSRPN25 were highly expressed in salivary glands with relative expression levels of 4.244 ± 1.39 and 74.44 ± 25.76 (P <0.01) The expression of AaSRPN17 and AaSRPN20 was the highest in the gut, with relative expression levels of 1.80 ± 0.33 and 0.703 ± 0.501, respectively. The relative expression levels of AaSRPN17 and AaSRPN20 were 0.34 ± 0.09 and 0.15 ± 0.03, respectively. Conclusion A total of 26 serpin genes were screened from the Aedes aegypti genome. Among them, 19 had signal peptide, and 16 were presumed to inhibit the activity of the enzyme.