论文部分内容阅读
目的:建立以反相高效液相色谱法测定乳痛宁糖浆中淫羊藿苷含量的方法,同时对淫羊藿和柴胡的原料药进行鉴别。方法:以薄层色谱(TLC)法鉴别淫羊藿与柴胡的原料药;采用反相高效液相色谱(RP-HPLC)法测定乳痛宁糖浆中的淫羊藿苷含量。色谱柱为Spheri-5RP-C_(18)(250mm×4.6mm,5μm),流动相为乙腈-水(30:70);流速为1.0mL·min~(-1);检测波长为270nm。结果:淫羊藿苷检测浓度在2.97～107.10ug·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 7);平均加样回收率为101.33%,RSD=1.75%(n=9)。结论:本法简便快捷,灵敏度高,重现性好,可作为该制剂的质量控制。
Objective: To establish a method for the determination of icariin content in Rutongning syrup by reversed-phase high performance liquid chromatography. At the same time, identify the raw materials of Epimedium and Bupleurum. Methods: TLC was used to identify the drug substance of epimedium and Bupleurum chinense; RP-HPLC was used to determine the content of icariin in Rutongning syrup. The chromatographic column was Spheri-5RP-C_(18)(250mm×4.6mm, 5μm), the mobile phase was acetonitrile-water(30:70), the flow rate was 1.0mL·min~(-1), and the detection wavelength was 270nm. Results: In the range of 2.97-107.10 ug.mL-1, the icariin detection concentration showed a good linear relationship with the peak area (r=0.999 7); the average recovery was 101.33%, and the RSD was 1.75% (n =9). Conclusion: This method is simple and rapid, with high sensitivity and good reproducibility. It can be used as the quality control of the preparation.