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目的:主要探讨原代心房肌细胞的培养及鉴定方法,为进一步研究心房颤动的重构机制及治疗方法奠定基础。方法:选取1-3 d的SD乳鼠40只,雌雄不限,分离心房、心室肌,胰酶联合EDTA充分消化心房肌细胞,利用心房肌与成纤维细胞的差速贴壁及细胞传代方法纯化心房肌细胞,免疫细胞化学染色鉴定心房肌细胞。结果:心房肌细胞培养至第3天,可见心房肌细胞覆盖率高达90%,并出现波动性,免疫细胞化学染色可见90%的心房肌细胞肌经α-肌动蛋白抗体染色阳性。结论:经酶化学消化法可成功培养出原代心房肌细胞,是一种较好的培养及鉴定乳鼠心房肌细胞的方法。
Objective: To study the methods of primary atrial myocyte cultivation and identification, and to lay the foundation for further research on the mechanism of atrial fibrillation remodeling and treatment. Methods: Forty SD neonatal rats (1-3 days) were randomly divided into three groups: atrial and ventricular myocardium, pancreatic enzyme and EDTA. The atrial myocytes were digested with EDTA. The differential adherence of atrial and fibroblasts and the passage of cells Atrial myocytes were purified and immunocytochemistry was used to identify atrial myocytes. Results: Atrial myocytes were cultured on the third day. The coverage of atrial myocytes was as high as 90%, and the fluctuation of the atrial myocytes was observed. Immunocytochemical staining showed that 90% of the atrial myocytes were stained positive by α-actin antibody. Conclusion: The primary atrial myocytes can be successfully cultured by enzymatic digestion, which is a good method for culturing and identifying atrial myocytes in neonatal rats.