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2011~2014年间,本研究采用人工点授和控制授粉两种方式,以紫娘喜、无核荔、白糖罂、妃子笑、三月红、新球蜜荔6个荔枝品种为亲本进行杂交,创制了11个杂交组合,并利用5对SSR标记和3对In Del标记引物从2 317株F1代中鉴定真杂种1 666株。结果显示:不同组合杂交效率存在差异,平均真杂种率为71.9%;杂交后代中发现了双亲位点缺失、新位点出现的现象;发现了可用于紫娘喜×无核荔、无核荔×紫娘喜、紫娘喜×妃子笑、紫娘喜×三月红、无核荔×新球蜜荔、新球蜜荔×无核荔、白糖罂×无核荔、白糖罂×三月红8个组合后代真杂种鉴定的5个纯合显性标记。研究结果表明,人工点授和控制授粉两种方式的真杂种率没有显著差异。
From 2011 to 2014, six kinds of litchi varieties were crossed by artificial lodging and controlling pollination in this research. The results showed that the two litchi varieties were crossed with purple niangxi, non-nuclear Lai, white sugar poppy, concubine, March red, 11 hybrid combinations were created and 1 666 true hybrids were identified from 2 317 F1 progenies using 5 pairs of SSR markers and 3 pairs of In Del marker primers. The results showed that there were differences in the hybridization efficiency of different combinations, the average true true hybrid rate was 71.9%; hybrid progeny found in the absence of a new parent site, the phenomenon of new sites; × purple niang hi, purple niang hi × concubine laugh, purple niang hi × March red, non-nuclear Lai × new ball honey Lai, new ball honey Lai × non-core Lai, sugar poppy × non-nuclear Lai, sugar poppy × March Five homozygous dominant markers for the identification of true hybrids of 8 combinations of red. The results showed that artificial point granting and controlling pollination of the two ways of the true population no significant difference.