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目的:探讨α-干扰素对慢性乙型肝炎患者记忆性CD4+T细胞变化的影响及其机制。方法:100例慢性乙型肝炎患者相应的治疗时间采集全血标本,分离外周血单个核细胞,采用FC500流式细胞仪收集和分析外周血记忆性CD4+T细胞亚群。结果:1.在IFN-α治疗过程中,可引起CD4+TCM、CD4+TEM和CD4+TIM细胞比例的规律性变化,即CD4+TCM与CD4+TIM变化规律基本相反,而CD4+TIM与CD4+TEM变化规律基本一致,说明用药时CD4+TCM向CD4+TIM和CD4+TEM转化。2.用药36周时CD4+TCM的增加与CD4+T细胞一致,而停药后CD4+TCM的减少与CD4+T细胞也一致,说明IFN-α导致了CD4+TCM细胞的增殖,从而使CD4+T细胞数量增加。结论:慢性乙型肝炎病毒感染患者用IFN-α治疗时,可引起CD4+TCM、CD4+TEM和CD4+TIM的规律性变化,使得效应CD4+TEM细胞增加;同时,记忆性CD4+T细胞数量增加,其增加原因可能与CD4+TCM细胞数量的增加有关。
Objective: To investigate the effect of α-interferon on the changes of memory CD4 + T cells in patients with chronic hepatitis B and its mechanism. Methods: Whole blood samples of 100 patients with chronic hepatitis B were collected at the corresponding treatment time. Peripheral blood mononuclear cells were isolated. FCM flow cytometry was used to collect and analyze peripheral memory CD4 + T cell subsets. During IFN-α treatment, regular changes of CD4 + TCM, CD4 + TEM and CD4 + TIM cells were observed, that is, CD4 + TCM and CD4 + TIM changed basically in the same way, while CD4 + TIM and CD4 + TEM changes in the same pattern, indicating the use of CD4 + TCM to CD4 + TIM and CD4 + TEM conversion. 2. The increase of CD4 + TCM at 36 weeks was consistent with that of CD4 + T cells, while the decrease of CD4 + TCM after withdrawal was also consistent with that of CD4 + T cells, indicating that IFN-α led to the proliferation of CD4 + TCM cells, CD4 + T cells increased. CONCLUSIONS: The treatment of patients with chronic hepatitis B virus infection with IFN-α causes regular changes of CD4 + TCM, CD4 + TEM and CD4 + TIM, leading to an increase in effector CD4 + TEM cells. Meanwhile, memory CD4 + T cells The increase may be related to the increase of CD4 + TCM cells.