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低温、高盐和干旱胁迫严重影响作物的生长和产量。为了挖掘花生逆境胁迫相关功能基因,本研究以花生品种花育33号为试验材料,根据c DNA文库中已知的脱水素(dehydrin,Dhn)基因全长序列设计引物,通过RT-PCR克隆到该基因,并命名为AhDHN1。该基因编码框为384bp,编码128个氨基酸。序列分析表明该氨基酸序列含有两个保守的富含赖氨酸片段和一个保守的富含丝氨酸片段,表明该蛋白为K2S型脱水素。荧光定量PCR结果显示,AhDHN1基因在花生的叶片和根中对低温没有明显响应,对高盐和干旱胁迫则有明显响应,说明该基因可能参与了花生对高盐和干旱胁迫的适应性调控。本研究为阐明花生抗逆分子机理提供了理论基础,为花生抗逆分子育种提供了新的基因资源。
Low temperature, high salt and drought stress seriously affect crop growth and yield. In order to excavate the functional genes related to the stress of peanut stress, we designed the primers according to the full-length sequence of dehydrin (Dhn) gene known in the c DNA library, and then cloned it by RT-PCR This gene, and named AhDHN1. The gene encoding a 384bp encoding 128 amino acids. Sequence analysis showed that the amino acid sequence contained two conserved lysine-rich fragments and one conserved serine-rich fragment, indicating that the protein was K2S-type dehydratin. Fluorescent quantitative PCR results showed that AhDHN1 gene had no obvious response to low temperature and high salt and drought stress in peanut leaves and roots, indicating that this gene may be involved in the adaptive regulation of peanut to high salt and drought stress. This study provides the theoretical basis for elucidating the molecular mechanism of peanut resistance and provides new genetic resources for molecular breeding of peanut.