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仙客来是一种重要的观赏花卉,为了建立其稳定的快繁增殖体系并研究体胚变异,以仙客来种苗球茎为试材,进行组织培养。方法:经过初培养和多次继代培养,诱导产生胚性愈伤,并建立胚性细胞的悬浮培养体系;固体培养和悬浮培养产生的胚性细胞团转至无激素的培养基上,诱导产生体细胞胚,并根据体胚萌发状态不同对体胚变异进行统计。结果:悬浮培养和固体培养均获得了大量体细胞胚,但存在很大变异,固体培养基上得到10.9%的正常体胚,其体胚变异机率为89.1%,其中单极芽、单极根、无极球和不规则形各占2.6%,44.5%,32.8%,9.17%;悬浮培养得到6.25%的正常体胚,变异机率为93.75%,其中单极芽、单极根、无极球和不规则形各占1.05%,44.4%,34.4%,13.9%。结论:可以为仙客来通过体胚发生进行快繁提供技术参考,但体胚存在很大变异,为减少变异仍需进一步研究。
Cyclamen is an important ornamental flower. In order to establish its stable multiplication and multiplication system and to study the variation of somatic embryo, cyclamen seedling bulbs were used as experimental materials for tissue culture. Methods: After initial culture and multiple subcultures, induced embryogenic callus was induced, and suspension culture system of embryogenic cells was established. Embryogenic cell mass produced in solid culture and suspension culture was transferred to hormone-free medium and induced Produce somatic embryos, and according to the different embryo germination state of somatic embryo variation statistics. Results: A large number of somatic embryos were obtained in both suspension culture and solid culture, but there was a great variation. The rate of somatic embryo mutation was 89.1% in 10.9% of normal somatic embryos on solid medium, in which monopolar buds, monopolar roots , Respectively. The percentage of non-polar and irregular shapes was 2.6%, 44.5%, 32.8% and 9.17%, respectively. The normal somatic embryos with 6.25% suspension culture showed 93.75% chance of mutation, including monopolar buds, monopolar roots, The rules of the shape of each 1.05%, 44.4%, 34.4%, 13.9%. Conclusion: Cyclobalanopsis can provide technical reference for rapid propagation through somatic embryogenesis. However, there is a great variation in somatic embryos and further study is needed to reduce the variation.