论文部分内容阅读
目的:观察携带hIFN-γ基因的内皮祖细胞(endothelial progenetor cells carrying hIFN-γ,EPC-hIFN-γ)在肿瘤化疗后维持治疗的效果。方法:LoVo大肠癌细胞加入喜树碱-11(camptothecin-11,CPT-11),再分别加入hIFN-γ或(和)西妥苷单抗(cetuximab,C225),MTT法观察其对LoVo细胞的抑制作用。荷Lovo大肠癌细胞移植瘤裸鼠在给药CPT-11后,再给予EPCs-hIFN-γ或(和)C225,观察对肿瘤的抑制作用和对裸鼠生存期的影响。结果:在体外,肿瘤细胞中加入CPT-11后再加入hIFN-γ或(和)C225可进一步抑制肿瘤细胞的生长。荷瘤裸鼠在给予CPT-11 50 mg/kg后,分别给予EPCs-hIFN-γ、C225或者EPCs-hIFN-γ+C225,均可以进一步抑制肿瘤的生长[肿瘤平均体积(2 024.28±1 048.40)mm3vs(764.94±720.14)mm3、(233.85±186.97)mm3、(186.95±133.43)mm3、(163.9±173.39)mm3,P<0.05),均可进一步延长荷瘤裸鼠的生存期(中位生存期34.2 dvs39.4 d、44.5 d、48.5 d、51.3 d,P<0.05或P<0.01);其中以CPT-11+EPCs-hIFN-γ+C225的治疗效果最好。结论:EPCs-hIFN-γ用于化疗后维持治疗可以抑制肿瘤细胞生长,延长荷瘤小鼠的生存。
OBJECTIVE: To observe the effect of endothelial progenitor cells carrying hIFN-γ carrying hIFN-γ (EPC-hIFN-γ) on the maintenance of chemotherapy after chemotherapy. Methods: Human LoVo cells were treated with camptothecin-11 (CPT-11) and then added with hIFN-γ or cetuximab (C225) Inhibition. After the administration of CPT-11, EPCs-hIFN-γ or (and) C225 was administered to nude mice bearing Lovo colorectal cancer cell xenografts. The inhibitory effect on the tumor and the effect on the survival of nude mice were observed. RESULTS: In vitro, addition of hIFN-γ or (and) C225 to tumor cells after addition of CPT-11 further inhibited the growth of tumor cells. Tumor bearing nude mice were given EPCs-hIFN-γ, C225 or EPCs-hIFN-γ + C225 respectively after given CPT-11 50 mg / kg, which could further inhibit tumor growth [mean tumor volume (2 024.28 ± 1 048.40 ) were significantly higher than those in the control group (P <0.05). All of these could further prolong the survival of the tumor-bearing nude mice (median survival EPCs-hIFN-γ + C225 was the best, the best treatment was 34.2 dvs39.4 d, 44.5 d, 48.5 d, 51.3 d, P <0.05 or P <0.01) Conclusion: The EPCs-hIFN-γ used for maintenance therapy after chemotherapy can inhibit the growth of tumor cells and prolong the survival of tumor-bearing mice.