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单核苷酸多态性标记已在农作物研究中得到广泛应用并取得重大进展。为了便利棉花SNP(Single nucleotide polymorphism)标记的研究和应用,介绍了利用基因芯片、简化基因组测序、重测序等在棉花中开发SNP标记的方法 ,综述了SNP标记在棉花遗传图谱构建、数量位点的定位和分子标记辅助育种、基因组测序以及系统进化等研究中的应用。并对异源四倍体棉花中SNP标记开发时,同源序列位点和部分同源序列位点上的SNP标记辨别问题进行了系统探讨,对其快捷的开发、检测方式和在数量基因定位中的应用前景进行了展望。
Single nucleotide polymorphism markers have been widely used in crop research and made significant progress. In order to facilitate the research and application of single nucleotide polymorphism (SNP) markers in cotton, methods for developing SNP markers in cotton using gene chip, simplified genome sequencing and resequencing were introduced. The SNP markers in cotton genetic map construction, quantitative loci Localization and molecular marker-assisted breeding, genome sequencing and phylogenetic studies. In the process of SNP markers development in heterosexual tetraploid cotton, the SNP markers identification at the homologous sequence sites and homologous sequence sites were systematically discussed. The rapid development and detection methods and the application of quantitative gene mapping In the application prospects of prospects.