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目的:比较上皮性钙黏蛋白(E-cadherin,E-cad)在人输卵管妊娠种植与非种植部位组织中的表达水平。方法:14例未破裂型输卵管壶腹部妊娠组织标本,均包括种植和非种植部位的组织,分为种植部位组(n=14)和非种植部位组(n=14);并以12例因其它疾患行全子宫加附件切除术的人分泌期正常输卵管壶腹部组织新鲜标本为对照组。采用免疫组织化学法观察E-cad蛋白在输卵管妊娠组织和输卵管黏膜上皮的表达情况,应用荧光定量聚合酶链反应(qRT-PCR)和免疫印迹法(Western blotting)检测其E-cadmRNA和蛋白表达水平。结果:E-cad蛋白表达于人输卵管黏膜上皮纤毛细胞和分泌细胞的胞膜,在细胞之间的连接面表达较强,管腔面表达较弱,纤毛未见表达。在输卵管妊娠组织,E-cad表达于蜕膜细胞、绒毛细胞滋养层细胞和绒毛外滋养细胞的胞膜,绒毛外滋养细胞的阳性显色弱于绒毛细胞滋养层细胞。E-cadmRNA和蛋白在种植部位组的表达显著低于非种植部位组和对照组(P<0.05),非种植部位组与对照组之间表达无显著性差异(P>0.05)。结论:E-cad在输卵管妊娠种植部位表达水平降低,可能与输卵管妊娠发生有密切的联系。
Objective: To compare the expression of E-cadherin (E-cad) in the implanted and non-implantation sites of human tubal pregnancy. Methods: Fourteen non-ruptured ampulla of pregnancy ampulla were divided into three groups: implantation site group (n = 14) and non-implantation site group (n = 14) Other disorders line hysterectomy with attachment excision of secretory normal tubal ampulla tissue fresh specimens for the control group. The expression of E-cad protein in tubal pregnancy and tubal mucosa epithelium was observed by immunohistochemistry. The expression of E-cadherin and protein was detected by qRT-PCR and Western blotting Level. Results: E-cad protein was expressed in the cell membrane of ciliated epithelial cells and secretory cells of human oviduct mucosal epithelium. The expression of E-cad protein was stronger in the connective surface of cells and less in the luminal surface, but not in the cilia. In tubal pregnancy, E-cad expression in the decidual cells, villus trophoblast cells and extravillous trophoblast cell membrane, villous trophoblast cells positive staining is weaker than villous trophoblast cells. The expression of E-cad mRNA and protein in the implantation site group was significantly lower than that in the non-implantation site group and the control group (P <0.05). There was no significant difference between the non-implantation site group and the control group (P> 0.05). Conclusion: The expression of E-cad in the implantation site of tubal pregnancy is decreased, which may be closely related to the occurrence of tubal pregnancy.