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采用比色法、荧光和吸收光谱法研究了Ｎ－２，４－二羟基苯甲醛氨基葡萄糖希夫碱（ＤＧ）的铜（Ⅱ）、锌（Ⅱ）、钴（Ⅲ）、铁（Ⅲ）配合物（ＭＤＧ）对超氧阴离子自由基和ＤＮＡ的作用．结果表明：ＣｕＤＧ，ＦｅＤＧ和ＣｏＤＧ能显著抑制超氧离子自由基的生成，抑制能力ＣｕＤＧ＞ＦｅＤＧ＞ＣｏＤＧ＞ＺｎＤＧ．此外，配合物ＣｕＤＧ和ＣｏＤＧ可与ＤＮＡ有效结合，结合常数（Ｋ。）值分别为３．０３×１０４ｍｏｌ－１·Ｌ和１．０８×１０６ｍｏｌ－１·Ｌ，每１００个核苷酸的片段可提供结合位置数分别是１．７和０．６．
The contents of copper (Ⅱ), zinc (Ⅱ), cobalt (Ⅲ) and iron (Ⅲ) in N-2,4-dihydroxybenzaldehyde glucosamine Schiff base (DG) were studied by colorimetry, fluorescence and absorption spectroscopy. Effect of Complex (MDG) on Superoxide Anion Radicals and DNA. The results showed that CuDG, FeDG and CoDG could significantly inhibit the formation of superoxide anion radicals and inhibitory ability of CuDG> FeDG> CoDG> ZnDG. In addition, the complexes CuDG and CoDG bind efficiently to DNA with binding constant (K.) values of 3.03 × 104 mol-1 · L and 1.08 × 106 mol-1 · L, respectively, for every 100 nucleotides The number of available binding positions is 1.7 and 0.6, respectively.