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目的:探讨二烯丙基二硫(DADS)对胃癌细胞NADPH氧化酶活性的影响及其机制。方法:用DADS作用于胃癌AGS细胞后,检测细胞NADPH氧化酶活性与mi R-34a的表达,以及SrcGab1-Shp2通路的活性,期间采用不同的干预,分析NADPH氧化酶、mi R-34a、Src-Gab1-Shp2通路之间的关系。结果:DADS作用后,AGS细胞NADPH氧化酶活性以及mi R-34a表达均明显升高(均P<0.05),而DADS升高NADPH氧化酶活性的作用被添加Src激酶抑制剂PP2所取消;DADS或mi R-34a作用后,AGS细胞Src、Gab1、Shp2 m RNA的表达均明显降低(均P<0.05),且DADS作用后,AGS细胞中磷酸化Src、Gab1、Shp2蛋白水平明显降低(均P<0.05)。结论:DADS能升高NADPH氧化酶活性,该作用可能与其上调mi R-34a表达,从而抑制Src-Gab1-Shp2通路活性有关。
Objective: To investigate the effect of diallyl disulfide (DADS) on the activity of NADPH oxidase in gastric cancer cells and its mechanism. Methods: The expression of NADPH oxidase, the expression of mi R-34a and the activity of SrcGab1-Shp2 pathway in gastric cancer cell line AGS were detected by DADS. The effects of NADPH oxidase, mi R-34a, Src -Gab1-Shp2 pathway. Results: After DADS treatment, the activities of NADPH oxidase and mi R-34a in AGS cells were significantly increased (all P <0.05), whereas the effect of DADS on the activity of NADPH oxidase was abolished by the addition of Src kinase inhibitor PP2. DADS Or mi R-34a, the expression of Src, Gab1 and Shp2 m RNA in AGS cells were significantly decreased (all P <0.05). After DADS treatment, the phosphorylated Src, Gab1 and Shp2 protein levels in AGS cells were significantly decreased P <0.05). Conclusion: DADS can increase the activity of NADPH oxidase, which may be related to the up-regulation of mi R-34a expression and the inhibition of Src-Gab1-Shp2 pathway activity.