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目的探讨低水平长期铅作用对雄性大鼠睾丸组织中抑制素B(inhibin B,INH B)亚基表达的影响。方法将12只9周龄SPF级SD雌性大鼠随机分为3组,分别为对照(去离子水)组和低剂量(800 mg/L)、高剂量(1 500 mg/L)乙酸铅染毒组。采用自由饮水方式连续染毒10 d后,按2∶1雌雄同笼。将孕鼠继续分笼喂养,孕期及哺乳期喂养方式同上。仔鼠断乳后,每组选择15只雄性仔鼠,对应分别给予0(去离子水)、300、900 mg/L乙酸铅溶液继续饮水染毒至6月龄。测定血铅和睾丸铅含量及睾丸组织抑制素Bα和βB亚基基因mRNA和蛋白的表达水平。结果与对照组相比,低、高剂量铅染毒组大鼠血铅和睾丸组织中铅含量均较高,而睾丸组织中抑制素Bα和βB亚基mRNA及蛋白的表达水平均下降,差异有统计学意义(P<0.05)。且随着铅染毒剂量的升高,大鼠血铅和睾丸组织中铅含量均呈上升趋势,而大鼠睾丸组织中INH Bα和βB mRNA及蛋白的表达水平均呈下降趋势。结论长期低剂量铅作用所致抑制素Bα和βB亚基表达下降可能参与了铅的雄性生殖毒性过程。
Objective To investigate the effect of low level long-term lead on the expression of inhibin B (INH B) subunit in testis of male rats. Methods Twelve female Sprague-Dawley (SD) SD rats of 9 weeks old were randomly divided into three groups: control (deionized water) group and low dose (800 mg / L) and high dose Poisonous group. Continuous drinking water for 10 d, according to 2: 1 male and female cage. The pregnant rats continue cage feeding, pregnancy and lactation feeding Ibid. After the weaned mice were weaned, 15 male offspring were selected in each group, corresponding to 0 (deionized water) respectively. The 300,900 mg / L lead acetate solution was continuously exposed to water for 6 months. The levels of lead and testicular lead in blood and the expression of testis tissue inhibitor of Bα and βB subunit mRNA and protein were determined. Results Compared with the control group, the levels of Pb and Pb in the blood lead and testis were significantly lower in the low and high dose lead-exposed groups, while the mRNA and protein expressions of inhibin Bα and βB subunit in the testis decreased There was statistical significance (P <0.05). With the increase of lead exposure dose, the content of lead in blood lead and testicular tissue of rats increased, while the expression of INH Bα and βB mRNA and protein in testis tissue decreased. Conclusion The decreased expression of inhibin Bα and βB subunits may be involved in the male reproductive toxicity of lead.