我们曾发现在中毒剂量梭曼处理的神经肌接头上,间接强直刺激诱发出以终板电位(EPP)消失为特征的终板区再生性去极化(RD),产生RD的机理尚未阐明,本文报道在同样条件下,微电泳外源性乙酰胆碱(ACh)诱发出类似RD的终板区再生性去极化。 结果表明,在正常条件下,于终板区微电泳外源性ACh(10ms×180nA,滞留电流为20nA),可记录到乙酰胆碱电位(AChP),0.5～2Hz串微电泳,相对应每次微电泳有一个AChP,高于5～10Hz(持续1～5s)的串 We have found that the mechanism by which indirect tetanic stimulation induces regenerative depolarization (RD) of the terminal plate area characterized by the disappearance of endplate potential (EPP) at the toxic dose of soman-treated neuromuscular junction has not yet been elucidated, We report here that under the same conditions, exogenous acetylcholine (ACh) by micro-electrophoresis induces regenerative depolarization of end-plate like RD. The results showed that AChP and 0.5 ~ 2Hz serial microarray can be recorded under the normal conditions in ex vivo ACh (10ms × 180nA, 20nA) Electrophoresis has an AChP, higher than 5 ~ 10Hz (lasting 1 ~ 5s) string