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目的:建立吴茱萸汁的质量标准。方法:采用薄层色谱法(TLC)对吴茱萸汁进行定性鉴别,采用高效液相色谱法测定吴茱萸内酯、吴茱萸碱、吴茱萸次碱的含量,Agilent C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水-四氢呋喃-冰醋酸(40∶60∶1∶0.2)为流动相,流速1 mL·min-1,柱温30℃,吴茱萸内酯、吴茱萸碱检测波长为225 nm、吴茱萸次碱检测波长为343nm。结果:薄层鉴别专属性强,重复性良好。吴茱萸内酯,吴茱萸碱和吴茱萸次碱分别在0.12～8.10(r=0.999 9),0.028～1.80(r=0.999 8),0.012～0.75μg(r=0.999 9)线性关系良好。平均回收率和RSD依次为101.49%,2.21%;102.65%,1.51%;100.47%,2.42%(n=6)。结论:方法简便,可靠,可操作性强,适用于吴茱萸汁炮制辅料的质量控制。
Objective: To establish the quality standard of evodia juice. Methods: Evodia rutaecarpa juice was identified by TLC. The contents of evodiamine, evodiamine and rutaecarpine were determined by high performance liquid chromatography (HPLC) on an Agilent C18 column (4.6 mm × 250 mm, 5 μm) , Acetonitrile-water-tetrahydrofuran-glacial acetic acid (40:60:1:0.2) as the mobile phase at a flow rate of 1 mL · min-1. The detection wavelength was set at250 nm with evodiamine and evodiamine The detection wavelength is 343nm. Results: Thin layer identification is highly specific and reproducible. Evodiamine, evodiamine and rutaecarpine were good linearity between 0.12 and 8.10 (r = 0.999 9), 0.028-1.80 (r = 0.999 8) and 0.012-0.75 μg (r = 0.999 9) respectively. The average recovery and RSD were 101.49%, 2.21%, 102.65%, 1.51%, 100.47% and 2.42%, respectively (n = 6). Conclusion: The method is simple, reliable and practical. It is suitable for the quality control of the excipients for evodia juice processing.