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目的食管癌是全球常见的的恶性肿瘤,新疆为发病率较高的地区之一。本研究旨在完善新疆汉族食管鳞癌(esophageal squamous cell carcinoma,ESCC)基因表达谱,进一步了解ESCC的发生、发展机制,提供与ESCC的预防、诊断及治疗相关的新的生物标志。方法收集2014-11-03-2015-09-25新疆石河子大学医学院第一附属医院兵团内镜中心6例汉族中分化ESCC患者的癌组织和癌旁正常组织(距癌组织>5cm)。利用基因芯片技术检测新疆汉族ESCC的差异表达基因。结果共筛选出汉族ESCC差异表达的mRNAs 335个,其中有138个表达上调的mRNAs和197个表达下调的mRNAs,差异倍数(fold change,FC)≥2且P<0.01,这些基因涉及多种生物学功能及通路。结论利用基因芯片技术基于人类全基因谱筛选了新疆汉族ESCC差异表达基因,完善了新疆汉族ESCC基因谱,为ESCC的诊断及预后的进一步研究打下基础。
Purpose Esophageal cancer is a common malignant tumor in the world. Xinjiang is one of the regions with a high incidence. This study aims to improve the gene expression profile of esophageal squamous cell carcinoma (ESCC) in Xinjiang Han nationality and further understand the mechanism of ESCC development and provide new biomarkers related to the prevention, diagnosis and treatment of ESCC. Methods Six cases of moderately differentiated ESCC and adjacent normal tissues (> 5cm away from cancer) in the endoscopic center of Corps Hospital of the First Affiliated Hospital of Shihezi University School of Medicine in Xinjiang were collected from 2014-11-03-2015-09-25. Detection of differentially expressed genes in ESCC of Xinjiang Han using gene chip technology. Results A total of 335 ESTs were differentially expressed in ESCC of Han ethnicity, including 138 up-regulated mRNAs and 197 down-regulated mRNAs, with fold change (FC) ≥2 and P <0.01. These genes involved in multiple organism Learning function and access. Conclusion The cDNA microarray of ESCC in Xinjiang Han ethnic group was screened by gene chip technology based on human whole gene spectrum and the ESCC gene profile of Xinjiang Han nationality was improved, which laid the foundation for the further study on the diagnosis and prognosis of ESCC.