【摘 要】
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The purpose of this study is to determine whether synapses can be formed between spinal motor neurons (SMNs)and major pelvic ganglion (MPG) neurons of a rat in vitro.The green fluorescent protein (GFP)-labelled MPG cells were cultured together with SMNs i
【机 构】
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Department of Surgery,Maternal and Child Health Hospital of Hubei Province,Tongji Medical College of
论文部分内容阅读
The purpose of this study is to determine whether synapses can be formed between spinal motor neurons (SMNs)and major pelvic ganglion (MPG) neurons of a rat in vitro.The green fluorescent protein (GFP)-labelled MPG cells were cultured together with SMNs in a specific medium.The synaptic-like con-tacts established between SMNs and MPG neurons were studied in co-cultures using morphologic and immunocytochemistry ap-proaches.Phase-contrast observation of co-cultures showed ap-parent SMNs-MPG neurons contacts as early as three or four days in vitro.We demonstrate some evidence of synaptic contacts be-tween SMNs and MPG neurons in vitro by immunostaining with antibody directed against postsynaptic density protein 95(PSD-95).We describe the development process of a defined SMNs-MPG neurons co-culture system.The results suggest that the hybrid synapse formation that may occur between SMNs and MPG neurons in vitro played an essential role in the mechanisms of a regenerated bladder with an artificial somatic-autonomic re-flex arc.
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