论文部分内容阅读
将人工杂交选育的坛紫菜新品系Z-26,Z-61,Z-81,Z-17的子3代(F3)与野生型对照组进行遗传性状的比较,旨在筛选耐高温品系。实验结果表明:(1)在高温29℃下培养40 d,人工选育的4个新品系丝状体均能正常生长发育,其中Z-26丝状体生长较快,Z-61在29℃下较易成熟,95%的营养藻丝发育成孢子囊枝,Z-17只有30%形成孢子囊枝;(2)Z-26和Z-61品系的叶状体在30℃下培养10 d,虽然藻体变白,但没有死亡,此时将其移至21℃下恢复培养3~4 d,藻体逐渐恢复为原有的色泽;(3)Z-81和Z-17的叶状体在29℃下培养7~10 d,虽然藻体发白但尚未死亡,移至21℃下培养基本可恢复正常,但在30℃下培养10 d,藻体腐烂不可恢复;(4)野生型对照组不耐高温,藻体在27℃下培养5 d就腐烂死亡,人工选育的4个品系耐受高温的幅度比野生型对照组高1~3℃;(5)4个品系叶状体在26~28℃温度下培养10 d后,其主要的藻胆蛋白和叶绿素a的含量与21℃培养下的含量差异不显著,说明短时间的高温培养并不影响其品质。
Comparison of the genetic traits of the third generation (F3) Zanthoxylum nitidifolius Z-26, Z-61, Z-81, and Z-17 selected by artificial breeding and the wild type control group was aimed at screening the high temperature resistant strains. The results showed as follows: (1) The four newly cultivated filamentous bodies grew normally at 40 ℃ for 40 days. The Z-26 filaments grew rapidly at 29 ℃ Under the mature conditions, 95% of the vegetative algae developed into sporocysts and only 30% of Z-17 formed sporangial branches. (2) The fronds of Z-26 and Z-61 lines were cultured at 30 ℃ for 10 days Although the algae were white, but did not die, at this time they were moved to 21 ℃ for 3 to 4 days, and the algae gradually returned to their original color. (3) Leaf shape of Z-81 and Z-17 The explants were cultured at 29 ℃ for 7-10 days. Although the algae were white but not dead, the culture returned to normal at 21 ℃. However, the culture at 30 ℃ for 10 days could not restore the decay of algae. (4) The control group was not tolerant to high temperature. The algal cells died after being cultured for 5 days at 27 ℃. The four strains of artificial selection tolerated the high temperature range of 1 ~ 3 ℃ higher than the wild type control. (5) After cultured for 10 days at 26-28 ℃, the contents of phycobiliprotein and chlorophyll a were not significantly different from those cultured at 21 ℃, which indicated that the short-term high-temperature culture did not affect its quality.