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为了探究广丰千金薯驯化移栽苗的耐旱生理机制,测定了干旱胁迫下广丰千金薯驯化移栽苗的多项光合生理指标。结果表明,50和100 g·L-1PEG-6000可显著促进广丰千金薯移栽驯化苗的生物量和根冠比,而150和200 g·L-1PEG-6000则显著减少其生物量和根冠比。当PEG-6000浓度为50和100 g·L-1,其叶片相对含水量、气孔开度、Tr、Fo、Fv/Fm、ΦPSⅡ、NPQ和ZR含量无显著变化,叶绿素含量、气孔密度、Pn、Gs、Ls、WUE、CUE、Fm、Fv/Fo、Fv’/Fm’、q P、GA3和ABA含量显著增加,Ci显著下降。当PEG-6000浓度达到150和200 g·L-1,其叶片相对含水量、叶绿素含量、气孔开度、Pn、Gs、Ls、Tr、WUE、CUE、Fo、Fm、Fv/Fo、ΦPSⅡ、Fv’/Fm’、Fv/Fm、q P、GA3、ZR和IAA显著下降,气孔密度、Ci、NPQ和ABA含量显著增加。在干旱胁迫下,广丰千金薯移栽驯化苗叶片ZR/ABA和ZR/IAA随着PEG-6000浓度的增大而显著下降,而ABA/(IAA+GA3+ZR)则随PEG-6000浓度的增大而显著提高。在干旱胁迫下广丰千金薯移栽驯化苗叶片倍性稳定,但当PEG-6000浓度达到200 g·L-1,电泳图谱上出现了明显的DNA ladder。因此,广丰千金薯驯化移栽苗能适应一定程度的干旱胁迫,但随着干旱胁迫的加剧,会影响其生长发育、光合作用和内源激素的调控机制,导致细胞程序性死亡。
In order to explore the physiological mechanism of tolerance to drought in the domestication of transplanted seedlings of Qianfang Qianjin, the photosynthetic physiological indices of domesticated transplanted seedlings of F. chinensis under drought stress were determined. The results showed that 50 and 100 g · L-1 PEG-6000 significantly promoted the biomass and root-shoot ratio of the transplanted domestication seedlings of F. chinense. However, 150 and 200 g · L-1 PEG-6000 significantly reduced the biomass and Root-crown ratio. The relative water content, stomatal opening, Tr, Fo, Fv / Fm, ΦPSⅡ, NPQ and ZR contents of PEG-6000 at 50 and 100 g · L-1 had no significant changes, and chlorophyll content, stomatal density, Pn Gs, Ls, WUE, CUE, Fm, Fv / Fo, Fv ’/ Fm’, q P, GA3 and ABA were significantly increased, Ci significantly decreased. The relative water content, chlorophyll content, stomatal opening, Pn, Gs, Ls, Tr, WUE, CUE, Fo, Fm, Fv / Fo and ΦPSⅡ of PEG-6000 reached 150 and 200 g · L- Fv / Fm, Fv / Fm, q P, GA3, ZR and IAA decreased significantly, stomatal density, Ci, NPQ and ABA content increased significantly. Under drought stress, ZR / ABA and ZR / IAA decreased significantly with the increase of PEG-6000 concentration, but ABA / (IAA + GA3 + ZR) decreased with PEG-6000 concentration Increase significantly increased. Under drought stress, the leaves of Guanghou Qianjin potato were ploidy and stable. However, when the concentration of PEG-6000 reached 200 g · L -1, the DNA ladder appeared on the electrophoresis pattern. Therefore, the transplanting seedlings of domestication can adapt to a certain degree of drought stress, but with the aggravation of drought stress, it will affect its growth and development, photosynthesis and regulation of endogenous hormones, leading to programmed cell death.