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目的探讨非小细胞肺癌(Non-Small CellLung Cancer,NSCLC)肿瘤间质淋巴管生成与临床病理因素之间的关系。方法应用抗Podoplanin单克隆抗体(Elivision法)对68例NSCLC术后石蜡切片进行染色,观察肿瘤间质和癌旁组织淋巴管密度(Lymphatic vessel density,LVD)。结果①癌旁组织中淋巴管密度平均值为7.50±0.21(3.5～11.4),肿瘤组织间质中LVD为11.91±0.60(4.5～21.5),两组相比差异有统计学意义(P<0.05);②按有无淋巴结转移分组,68例NSCLC肿瘤组织间质中淋巴结阳性患者LVD平均值为15.73±0.74,淋巴结阴性患者LVD平均值为8.31±0.32,两组相比差异有统计学意义(P<0.05);以(高分化+中分化)和(低分化+未分化)进行分组,其LVD分别为9.42±0.54和15.46±0.87,两组相比差异有统计学意义(P<0.05);以Ⅰ、Ⅱ和Ⅲa期进行分组,其LVD分别为8.21±0.32,15.19±1.04和15.20±1.01,各组内差异有统计学意义(P<0.05)。结论非小细胞肺癌组织中淋巴管密度在分期较晚的肿瘤和有淋巴结转移的肿瘤中显著增高,从而有望为NSCLC抗淋巴管生成的研究提供理论依据。
Objective To investigate the relationship between lymphangiogenesis and clinicopathologic factors in non-small cell lung cancer (NSCLC). Methods Sixty-eight paraffin sections of NSCLC were stained with anti-Podoplanin monoclonal antibody (Elivision method) to observe the lymphatic vessel density (LVD) in the stromal and para-cancerous tissues. Results ① The average lymphatic vessel density in adjacent tissues was 7.50 ± 0.21 (3.5 ~ 11.4), and the LVD in tumor interstitium was 11.91 ± 0.60 (4.5 ~ 21.5), with significant difference between the two groups (P <0.05 ); ② According to the presence or absence of lymph node metastasis group, 68 cases of NSCLC tumor interstitial lymph node-positive patients with LVD mean was 15.73 ± 0.74, lymph node negative patients with LVD average was 8.31 ± 0.32, the difference was statistically significant (P < (P <0.05). The LVDs were (9.42 ± 0.54) and (15.46 ± 0.87) respectively in the groups of (well differentiated + moderately differentiated) and (poorly differentiated + undifferentiated), with significant difference between the two groups The LVD of group Ⅰ, Ⅱ and Ⅲa were 8.21 ± 0.32, 15.19 ± 1.04 and 15.20 ± 1.01, respectively. There was significant difference among the groups (P <0.05). Conclusions Lymphatic vessel density in non-small cell lung cancer tissues is significantly higher in tumors with late stage and with lymph node metastases, which may provide a theoretical basis for the study of anti-lymphangiogenesis in NSCLC.