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目的建立测定克霉唑软膏中克霉唑(C22H17CIN2)含量的方法。方法HPLC法,以HypersilC18(4.6mm×250mm,5μm)为色谱住,磷酸二氢钾溶液(0.015mol·mL-1)(用磷酸调至pH为3.0)-甲醇(15∶85)为流动相,流速1.0ml·min-1,检测波长为215nm,进样量10μL。结果克霉唑线性范围为21.48μg~64.44μg,r=0.9999,平均回收率为99.32,n=9,重复性良好。结论本方法准确、简便、快捷,可用于测定克霉唑软膏中克霉唑的含量。
Objective To establish a method for the determination of clotrimazole (C22H17CIN2) in clotrimazole ointment. Methods The HPLC method was performed on a Hypersil C18 column (4.6 mm × 250 mm, 5 μm). Potassium dihydrogen phosphate (0.015 mol · mL -1) (adjusted to pH 3.0 with phosphoric acid) -methanol (15:85) , Flow rate 1.0ml · min-1, detection wavelength of 215nm, injection volume 10μL. Results Clotrimazole linear range of 21.48μg ~ 64.44μg, r = 0.9999, the average recovery was 99.32, n = 9, good reproducibility. Conclusion This method is accurate, simple and rapid, and can be used to determine the content of clotrimazole in clotrimazole ointment.