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目的:研究麦冬多糖对CCl4诱导的大鼠肝损伤的保护作用与作用机制.方法:将48只大鼠随机分为6组,每组8只,设定空白对照组,模型组,麦冬多糖3个剂量组分别为:高剂量组(400 mg/kg)、中剂量组(200 mg/kg)、低剂量组(100 mg/kg),阳性药采用联苯双酯(150 mg/kg).以2mg/kg的40％CCl4花生油溶液造模,测定大鼠脏器指数,同时测定大鼠血清中的丙氨酸氨基转移酶(AU)和天门冬氨酸氨基转移酶(AST)的水平,以及血清中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量,并且在显微镜下观察肝脏组织病理学变化.结果:麦冬多糖组丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性和血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的水平与模型对照组相比均具有显著性差异(P＜0.05);模型组血清中SOD的活性为(114.01±22.15),麦冬多糖400 mg/kg组为(222.16±29.17),组间差异有统计学意义(P＜0.01),模型组血清中MDA含量为(5.39±1.74),麦冬多糖400 mg/kg组为(1.93±0.39),组间差异有统计学意义(P＜0.01),减轻对肝脏细胞的病理损害.结论:麦冬多糖可以保护CC14所引导的急性肝损伤,进一步的研究发现其肝保护机制可能与抗氧化通路有关.“,”Objective:To study the effects of polysaccharides on CCl4-induced liver injury in rats.Method:The 48 rats were divided into 6 groups to set the blank control group,model group,polysaccharides groups (three doses groups) as follows:high-dose group(400 mg/kg),middle dose group(200 mg/kg) and low-dose group(100 mg/kg),positive control as drug bifendate(150 mg/kg).The rat models were established by 40％ CCl4 peanut solution at the does of 2 mg/kg to measure the organindex,serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) levels,serum superoxide dismutase (SOD) activity,malondialdehyde(MDA) content and liver tissue pathological changes were observed under a microscope.Results:The alanine aminotransferase (ALT),aspartate aminotransferase (AST) activity,serum superoxide dismutase(SOD),and malondialdehyde (MDA) level in polysaccharides group were significantly higher than that of the control group(P＜0.05).Moreover,the serum SOD activity in control group is (114.01±22.15),compared to that in polysaccharides 400 mg/kg group of (222.16±29.17) (P＜0.01).MDA content in model group serum was (5.39±1.74),polysaccharides 400 mg/kg group was (1.93±0.39) (P＜0.01),consistent with reduced the pathological damage to the liver cells.Conclusion:The polysaccharides could protect CC14-induced acute liver injury and further studies found that liver protective mechanism may be related to the antioxidant pathway.