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目的:实验以百华花楸提取物对DPPH清除率为指标,以保证提取物高抗氧化活性。方法:实验在单因素试验基础上,采用CCD响应面设计法,建立了乙醇浓度、料液比、超声温度三因素与DPPH抗氧化活性之间的回归优化模型,对超声波辅助提取工艺进行优化。结果:确定了最佳提取工艺参数为:乙醇浓度81%,料液比10∶51,超声温度52℃,对DPPH抗氧化活性的实际值为3328.79(μmolTE/100g)。结论:响应面法对于优化百华花楸提取物抗氧化活性的工艺合理可行,为增加提取物的抗氧化活性提供了理论依据。
OBJECTIVE: To determine the scavenging rate of DPPH to Sorghum bicolor extract in order to ensure its high antioxidant activity. Methods: On the basis of single factor experiments, a CCD response surface design method was used to establish a regression optimization model of three factors of ethanol concentration, solid-liquid ratio, ultrasonic temperature and DPPH antioxidant activity, and the ultrasonic assisted extraction process was optimized. Results: The optimal extraction parameters were: ethanol concentration 81%, solid-liquid ratio 10:51 and ultrasonic temperature 52 ℃. The actual value of DPPH antioxidant activity was 3328.79 (μmolTE / 100g). Conclusion: The response surface method is reasonable and feasible for optimizing the antioxidant activity of the extract of Baihua cathayzer, and provides a theoretical basis for increasing the antioxidant activity of the extract.