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文章介绍了3H同位素标记测定猪外周血白细胞介素-2活性的方法。此方法标记率高,重复性好,既可作短期(4h~6h)、又可作长时间(15h~24h)的细胞培养检测试验,还可用于收集仪收集制备细胞样品的检测试验。所使用的依赖大鼠因子(RF)的小鼠牌细胞对猪白细胞介素-2敏感,并呈剂量依赖关系。
The article introduced 3H isotope labeled porcine peripheral blood interleukin -2 activity. The method has the advantages of high labeling rate and good repeatability, and can be used for cell culture test in the short term (4h ~ 6h) and for long time (15h ~ 24h), and can also be used in collecting tests for collecting cell samples. The mouse factor-dependent (RF) -dependent mouse card cells used were sensitive to porcine interleukin-2 in a dose-dependent manner.